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Established in 2004, the Molecular Biology and Biochemistry Center (MBBC) is a College of Liberal Arts and Sciences research and teaching facility that consists of several laboratories and offices in the north-west corner of the third floor of Snygg Hall. The MBBC is comprised of faculty and students from the Departments of Biological Sciences and Chemistry. The MBBC is also adjacent to the NSF-funded Mass Spectrometry/Proteomics Center (MaSPeC). Any persons interested in using the facilities may contact any of the participating faculty listed below.
Faculty
Kestas Bendinskas (Chemistry)
Research interests in the purification of GHB specific enzymes and the studies of effects of drugs on insulin producing pancreatic cells
Tim Braun (Biological Sciences)
Research interests in bacterial motility and bacterial physiology with special interest in bioenergetics
Webe Kadima (Chemistry)
Research interests in the binding of biological ligands and the effects of metal ions in the confomational transition to the insulin hexamer using NMR and static and dynamic light scattering studies
Jim MacKenzie (Biological Sciences)
Research interests in protein trafficking to mitochondria, environmental toxicants and cardiovascular reactivity in children
Peter Rosenbaum (Biological Sciences)
Research interests in genetic epidemiology, evolutionary processes, and herpetology
Amy Welsh (Biological Sciences)
Research interests in conservation of endangered species, native fish conservation, and wildlife forensics
Students
Current Students
Amanda Hewlett (Biology)
Randall Smith (Zoology)
Zach Zebrowski (Biology)
Former Students
Matt Arthur (Biology)
Andrew Banyikwa (Chemistry, Graduate Student)
Kristen Belitz (Biology)
Robert Birdsall (Chemistry, Graduate Student)
Amy Boleto (Biology)
Jenna Burgess (Zoology)
Jennifer Bushey (Zoology)
Katy Cook (Biochemistry)
Dan Crosset (Biology)
Dan Dempsey (Chemistry, Graduate Student)
Sarah Faddegon (Zoology)
Kathy Gebbie (Zoology)
Danielle Gilbert (Chemistry, Graduate Student)
Thea Hassan (Biology)
John Heagerty (Biology)
Tia Hendershott (Biology)
Mark Hudson (Biology)
Mike Kiley (Biochemistry)
Paul MacMahon (Biochemistry)
Tabitha Maier (Chemistry)
Mike Mastromauro (Zoology)
John Merchant (Chemistry, Graduate Student)
Greg Miller (Biochemistry)
Katie Miloski (Chemistry, Graduate Student)
Zach Neyhard (Adolescent Education, Biology)
Anders Peterson (Biology)
Frank Pierce (Biology)
Merideth Pritchard (Chemistry)
Kristen Roosa (Biology)
Patricia Sattelberg (Biochemistry)
Erin Simon (Chemistry, Graduate Student)
Cody Spencer (Biology)
Kelly Wallace (Biology)
Beth Whitmore (Zoology)
Eric Yeager (Zoology)
Expertise
DNA – DNA sequencing, chromosomal and plasmid purification, agarose and polyacrylamide gel electrophoresis, restriction analysis, PCR, site-directed mutagenesis, cDNA cloning, transformation of competent cells, DNA polymorphism analysis, DNA labeling
RNA – purification, agarose gel electrophoresis, Northern analysis, RT-PCR, in vitro transcription, RNA sequence analysis
Protein – expression, purification, SDS-PAGE, Western analysis, 2-D PAGE, enzyme kinetics, in vitro translation, radiolabeled competition analysis, MALDI-TOF analysis, inhibition studies, coupled enzymatic reactions
Cell biology – mammalian cell culture, bacterial cell culture, apoptosis studies, cytotoxicity screens (growth inhibition/cell kill, IC50), subcellular fractionation, intracellular protein trafficking
Computer skills – DNA and protein sequence analysis (secondary structure prediction, specific motif identification, database searches, and hydropathy, similarity and amphipathicity analyses) using SeqWeb and bioinformatics web sites (NCBI, SwissProt, Expasy, etc.), 2-D PAGE analysis software (ImageMaster), Statistics Programs (SPSS, GraphPad), Adobe Photoshop
Major Equipment
Sorvall WX80 ultracentrifuge
Beckman high-speed centrifuges (2)
Bio-TEK microplate reader and plate washer
Bio Rad DCode Universal Mutation Detection System
-80°C freezers
Biosafety Level 2 hoods
Scintillation counter
Kodak gel documentation system
Other Equipment
Water, gas, and air are all on line. Adequate bench space, sinks, and fume hoods for hazardous volatile materials are also present. The research lab is equipped with microfuges, PAGE and agarose electrophoresis equipment, electrophoresis power supplies, gel drying system, thermocyclers, UV crosslinker, bacterial shaking incubators (4-80°C), hybridization oven, Spectronic UV/Vis spectrophotometer, dissolved oxygen monitor, analytical balances, and a vacufuge. Complete and separate work areas for mammalian cell culture and work with radioisotopes are also available. Adjacent to the MBBC is a 4°C walk-in refrigerated room and an autoclave.
Please report dead links to james.mackenzie@oswego.edu
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